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- Title
A biobanking turning‐point in the use of formalin‐fixed, paraffin tumor blocks to unveil kinase signaling in melanoma.
- Authors
Velasquez, Erika; Szadai, Leticia; Zhou, Qimin; Kim, Yonghyo; Pla, Indira; Sanchez, Aniel; Appelqvist, Roger; Oskolas, Henriett; Marko‐Varga, Matilda; Lee, Boram; Kwon, Ho Jeong; Malm, Johan; Szász, Attila Marcell; Gil, Jeovanis; Betancourt, Lazaro Hiram; Németh, István Balázs; Marko‐Varga, György
- Abstract
A biobanking turning-point in the use of formalin-fixed, paraffin tumor blocks to unveil kinase signaling in melanoma (C) Quantification of the phosphosite Y187 in a patient with BRAFV600E positive tumor metastasis compared to a patient with WT-BRAF tumor metastasis for both FFPE and FFT samples. Abbreviations BRAF serine/threonine-protein kinase B-raf ERK extracellular signal-regulated kinase FFPE formalin-fixed and paraffin-embedded FFT fresh frozen tumors MEK mitogen-activated protein kinase MM malignant melanoma MS mass spectrometry PTMs posttranslational modifications Malignant melanoma (MM) is one of the most aggressive human solid tumors, and it is associated with the highest mortality of all skin cancers.1 Aberrant patterns of protein expression and posttranslational modifications (PTMs) have been linked to MM pathogenesis,2 which has promoted large-scale proteomics studies. Blue and red colors represent BP up- and downregulated in primary tumors (respectively), regardless of condition gl Since alterations in protein phosphorylation most likely reflect changes in kinase activity levels, we assess the potential of FFPE samples to explore the MM kinome.
- Subjects
MELANOMA; PARAFFIN wax; PROTEOMICS; THERAPEUTICS; POST-translational modification; EXTRACELLULAR signal-regulated kinases
- Publication
Clinical & Translational Medicine, 2021, Vol 11, Issue 8, p1
- ISSN
2001-1326
- Publication type
Article
- DOI
10.1002/ctm2.466