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- Title
Vascular endothelial growth factor (VEGF-C1)-dependent inflammatory response of podocytes in nephrotic syndrome glomerulopathies in children: an immunohistochemical approach.
- Authors
Ostalska-Nowicka, D.; Zachwieja, J.; Nowicki, M.; Kaczmarek, E.; Siwinska, A.; Witt, M.
- Abstract
Ostalska-Nowicka D, Zachwieja J, Nowicki M, Kaczmarek E, Siwinska A&Witt M(2005)Histopathology46,176–183Vascular endothelial growth factor (VEGF-C1)-dependent inflammatory response of podocytes in nephrotic syndrome glomerulopathies in children: an immunohistochemical approach: To analyse expression and distribution of vascular endothelial growth factor (VEGF-C1), podocalyxin and synaptopodin within renal tissue in nephrotic syndrome glomerulopathies in children.: Renal biopsies performed at the time and in the manner recommended by the World Health Organization. The study group consisted of submicroscopic glomerulonephritis (n = 10), diffuse mesangial proliferation (n = 14) and focal segmental glomerulosclerosis (n = 5). The control tissue consisted of macroscopically normal appearing cortex taken from kidneys resected for localized neoplasms (n = 3). Material for immunohistochemistry was fixed in Bouin's solution and embedded in paraffin. Indirect immunohistochemistry using monoclonal anti-human antibodies directed against VEGF-C1, podocalyxin and synaptopodin was employed. The distribution of markers was quantified by computerized image analysis. In non-sclerosed glomeruli (within podocyte cytoplasm), VEGF-C1 was more expressed in podocytes of all groups (P < 0.0002), while the distribution of synaptopodin was less expressed in all groups (P < 0.0002). There was no statistical difference between all groups in the expression of podocalyxin.: The increased permeability of the filtration barrier in steroid-resistant glomerulopathies may be a consequence of subcellular changes in podocytes resulting from decreased expression of synaptopodin. Moreover, impaired permeability of endothelium could be secondary to increased expression of podocyte-derived VEGF-C1.
- Subjects
VASCULAR endothelial growth factors; GROWTH factors; NEPHROTIC syndrome in children; KIDNEY diseases; IMMUNOHISTOCHEMISTRY; HISTOPATHOLOGY; PEDIATRIC nephrology; PATHOLOGY
- Publication
Histopathology, 2005, Vol 46, Issue 2, p176
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/j.1365-2559.2005.02076.x