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- Title
Horka<sup>D</sup>, a Chromosome Instability-Causing Mutation in Drosophila, Is a Dominant-Negative Allele of lodestar.
- Authors
Szalontai, Tamas; Gaspar, Imre; Belecz, Istvan; Kerekes, Iren; Erdelyi, Miklos; Boros, Imre; Szabad, Janos
- Abstract
Correct segregation of chromosomes is particularly challenging during the rapid nuclear divisions of early embryogenesis. This process is disrupted by HorkaD, a dominant-negative mutation in Drosophila melanogaster that causes female sterility due to chromosome tangling and nondisjunction during oogenesis and early embryogenesis. HorkaD also renders chromosomes unstable during spermatogenesis, which leads to the formation of diplo//haplo mosaics, including the gynandromorphs. Complete loss of gene function brings about maternal-effect lethality: embryos of the females without the HorkaD-identified gene perish due to disrupted centrosome function, defective spindle assembly, formation of chromatin bridges, and abnormal chromosome segregation during the cleavage divisions. These defects are indicators of mitotic catastrophe and suggest that the gene product acts during the meiotic and the cleavage divisions, an idea that is supported by the observation that germ-line chimeras exhibit excessive germ-line and cleavage function. The gene affected by the HorkaD mutation is lodestar, a member of the helicase-related genes. The HorkaD mutation results in replacement of Ala777 with Thr, which we suggest causes chromosome instability by increasing the affinity of Lodestar for chromatin.
- Subjects
DROSOPHILA genetics; DROSOPHILA melanogaster; ANIMAL mutation; ANIMAL genetics; GENETIC mutation; CHROMOSOMES; GENETICS
- Publication
Genetics, 2009, Vol 181, Issue 2, p367
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.108.097345