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- Title
Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation.
- Authors
Dehelean, Cristina A.; Şoica, Codruţa; Ledeţi, Ionuţ; Aluaş, Mihaela; Zupko, Istvan; Găluşcan, Atena; Cinta-Pinzaru, Simona; Munteanu, Melania
- Abstract
Background: Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition. Results: New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice. Conclusions: The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts.
- Subjects
BETULIN; TRITERPENES; BIRCH; CANCER cells; SPECTRUM analysis; SPECTROMETRY
- Publication
Chemistry Central Journal, 2012, Vol 6, Issue 1, p137
- ISSN
1752-153X
- Publication type
Article
- DOI
10.1186/1752-153X-6-137