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- Title
Alterations in Helicobacter pylori outer membrane and outer membrane vesicle-associated lipopolysaccharides under iron-limiting growth conditions.
- Authors
Keenan, Jacqueline I.; Davis, Kylie A.; Beaugie, Clare R.; McGovern, Joseph J.; Moran, Anthony P.
- Abstract
Outer membrane vesicles (OMVs) shed from the gastroduodenal pathogen Helicobacter pylori have measurable effects on epithelial cell responses. The aim of this study was to determine the effect of iron availability, and its basis, on the extent and nature of lipopolysaccharide (LPS) produced on H. pylori OMVs and their parental bacterial cells. Electrophoretic, immunoblotting and structural analyses revealed that LPSs of bacterial cells grown under ironlimited conditions were notably shorter than those of bacteria and OMVs obtained from iron-replete conditions. Structural analysis and serological probing showed that LPSs of iron-replete cells and OMVs expressed O-chains of Lewisx with a terminal Lewisy unit, whereas Lewisy expression was notably reduced on bacteria and OMVs from ironlimiting conditions. Unlike the O-chain, the core oligosaccharide and lipid Amoieties of iron-replete and iron-limited bacteria and their OMVs were similar. Quantitatively, shed OMVs from iron-replete bacteria were found to be LPS-enriched, whereas shed OMVs from iron-limited bacteria had a significantly reduced content of LPS. These differences were linked to bacterial ATP levels. Since iron availability affects the extent and nature of LPS expressed by H. pylori, host iron status may contribute to H. pylori pathogenesis.
- Subjects
HELICOBACTER pylori; ENDOTOXINS; PATHOGENIC microorganisms; IMMUNOBLOTTING; ELECTROPHORETIC deposition; OLIGOSACCHARIDES; LIPIDS; GASTRIC mucosa
- Publication
Innate Immunity, 2008, Vol 14, Issue 5, p279
- ISSN
1753-4259
- Publication type
Article
- DOI
10.1177/1753425908096857