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- Title
Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor.
- Authors
Im, Hyungsoon; Shao, Huilin; Park, Yong Il; Peterson, Vanessa M; Castro, Cesar M; Weissleder, Ralph; Lee, Hakho
- Abstract
Exosomes show potential for cancer diagnostics because they transport molecular contents of the cells from which they originate. Detection and molecular profiling of exosomes is technically challenging and often requires extensive sample purification and labeling. Here we describe a label-free, high-throughput approach for quantitative analysis of exosomes. Our nano-plasmonic exosome (nPLEX) assay is based on transmission surface plasmon resonance through periodic nanohole arrays. Each array is functionalized with antibodies to enable profiling of exosome surface proteins and proteins present in exosome lysates. We show that this approach offers improved sensitivity over previous methods, enables portable operation when integrated with miniaturized optics and allows retrieval of exosomes for further study. Using nPLEX to analyze ascites samples from ovarian cancer patients, we find that exosomes derived from ovarian cancer cells can be identified by their expression of CD24 and EpCAM, suggesting the potential of exosomes for diagnostics.
- Subjects
EXOSOMES; PLASMONIC Raman sensors; CANCER diagnostic equipment; SURFACE plasmon resonance; PROTEIN research; CANCER cells
- Publication
Nature Biotechnology, 2014, Vol 32, Issue 5, p490
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt.2886