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- Title
Identification of de novo germline mutations and causal genes for sporadic diseases using trio‐based whole‐exome/genome sequencing.
- Authors
Jin, Zi‐Bing; Cai, Xue‐Bi; Li, Zhongshan; Liu, Zhenwei; Jiang, Yi; Wu, Jinyu
- Abstract
ABSTRACT: Whole‐genome or whole‐exome sequencing (WGS/WES) of the affected proband together with normal parents (trio) is commonly adopted to identify de novo germline mutations (DNMs) underlying sporadic cases of various genetic disorders. However, our current knowledge of the occurrence and functional effects of DNMs remains limited and accurately identifying the disease‐causing DNM from a group of irrelevant DNMs is complicated. Herein, we provide a general‐purpose discussion of important issues related to pathogenic gene identification based on trio‐based WGS/WES data. Specifically, the relevance of DNMs to human sporadic diseases, current knowledge of DNM biogenesis mechanisms, and common strategies or software tools used for DNM detection are reviewed, followed by a discussion of pathogenic gene prioritization. In addition, several key factors that may affect DNM identification accuracy and causal gene prioritization are reviewed. Based on recent major advances, this review both sheds light on how trio‐based WGS/WES technologies can play a significant role in the identification of DNMs and causal genes for sporadic diseases, and also discusses existing challenges.
- Subjects
GENETIC mutation; CEREBRAL amyloid angiopathy; HEMOGLOBIN polymorphisms; GENOMES; EXOMES
- Publication
Biological Reviews, 2018, Vol 93, Issue 2, p1014
- ISSN
1464-7931
- Publication type
Article
- DOI
10.1111/brv.12383