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- Title
V76D mutation in a conserved gD-crystallin region leads to dominant cataracts in mice.
- Authors
Graw, Jochen; Löster, Jana; Soewarto, Dian; Fuchs, Helmut; Reis, André; Wolf, Eckhard; Balling, Rudi; de Angelis, Martin Hrabé
- Abstract
During a large-scale ENU mutagenesis screen, a mouse mutant with a dominant cataract was detected and referred to as Aey4. Aim of this study was the morphological description of the mutant, the mapping of the mutation, and the characterization of the underlying molecular lesion. The slit-lamp examination revealed a strong nuclear cataract surrounded by a homogeneous milky opacity in the inner cortex. The histological analysis demonstrated remnants of cell nuclei throughout the entire lens. The mutation was mapped to Chromosome 1 by a genome-wide linkage making the six g-crystallin encoding genes and the closely linked bA2-crystallin encoding gene to relevant candidate genes. Finally, a T?A exchange in exon 2 of the gD-crystallin encoding gene (symbol: Crygd) was demonstrated to be causative for the cataract phenotype; this particular mutation is, therefore, referred to CrygoAey4. The alteration in codon 76 leads to an amino acid exchange of Val?Asp. Val at this position is highly conserved; it is found in all mouse and rat gD/E/F-crystallins as well as in the human gA- and gD-crystallins. It may be replaced solely by Ile, which is present in all bovine g-crystallins, in the rat and mouse gA/B/C-crystallins, as well as in the human gB/C-crystallins. It is predicted that the exchange of a hydrophobic side chain by a polar and acidic one might influence the microenvironment by a dramatic decrease of the isoelectric point by 1.5 pH units in the 10 amino acids surrounding position 76. The CrygdAey4 additionally demonstrates the importance of the integrity of the Cryg gene cluster for lens transparency.
- Subjects
GENETIC mutation; GENETICS; GENES; IMINO acids; RADIOGENETICS; ORGANELLES
- Publication
Mammalian Genome, 2002, Vol 13, Issue 8, p452
- ISSN
0938-8990
- Publication type
Article
- DOI
10.1007/s00335-002-3021-6