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- Title
Comparison of phenotypic and genotypic tropism determination in triple-class-experienced HIV patients eligible for maraviroc treatment.
- Authors
Vandekerckhove, Linos; Verhofstede, Chris; Demecheleer, Els; De Wit, Stephane; Florence, Eric; Fransen, Katrien; Moutschen, Michel; Mostmans, Wendy; Kabeya, Kabamba; Mackie, Nicola; Plum, Jean; Vaira, Dolores; Baelen, Kurt Van; Vandenbroucke, Ina; Eygen, Veerle Van; Marck, Herwig Van; Vogelaers, Dirk; Geretti, Anna Maria; Stuyver, Lieven J.
- Abstract
Background Determination of HIV-1 tropism is a pre-requisite to the use of CCR5 antagonists. This study evaluated the potential of population genotypic tropism tests (GTTs) in clinical practice, and the correlation with phenotypic tropism tests (PTTs) in patients accessing routine HIV care. Methods Forty-nine consecutive plasma samples for which an original TrofileTM assay was performed were obtained from triple-class-experienced patients in need of a therapy change. Viral tropism was defined as the consensus of three or more tropism calls obtained from the combination of two independent population PTT assays (Trofile Biosciences, San Francisco, CA, USA, and Virco, Beerse, Belgium), population GTTs and GTTs based on ultra-deep sequencing. If no consensus was reached, a clonal PTT was performed in order to finalize the tropism call. This two-step approach allowed the definition of a reference tropism call. Results According to the reference tropism result, 35/49 samples were CCR5 tropic (R5) (patients eligible for maraviroc treatment) and 14/49 were assigned as non-R5 tropic. The non-R5 samples [patients not eligible for maraviroc treatment according to the FDA/European Medicines Agency (EMEA) label] group included both the CXCR4 (X4) samples and the dual and mixed CCR5/CXCR4 (R5/X4) samples. Compared with TrofileTM population PTTs, population GTTs showed a higher sensitivity (97%) and a higher negative predictive value (91%), but almost equal specificity and an equal positive predictive value. Conclusions In line with recent reports from clinical trial data, our data support the use of population genotypic tropism testing as a tool for tropism determination before the start of maraviroc.
- Subjects
TROPISMS; HIV; FIRE assay; ANTIRETROVIRAL agents; MARAVIROC (Drug); THERAPEUTICS
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2011, Vol 66, Issue 2, p265
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkq458