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- Title
Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content.
- Authors
Yang Yang; Zhimin Cheng; Wanfeng Zhang; Wei Hei; Chang Lu; Chunbo Cai; Yan Zhao; Pengfei Gao; Xiaohong Guo; Guoqing Cao; Bugao Li
- Abstract
Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation. Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content. Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p< 0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content. Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.
- Subjects
ASPARTATE aminotransferase; FAT cells; MALATE dehydrogenase; NICOTINAMIDE adenine dinucleotide phosphate; LIPID metabolism
- Publication
Animal Bioscience, 2022, Vol 35, Issue 2, p155
- ISSN
2765-0189
- Publication type
Article
- DOI
10.5713/ab.21.0174