We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Identification of Colocasia esculenta(L.) Schott var. Lipu Taro leaf blight disease causal pathogen and its protoplasts preparation.
- Authors
DONG Wei-qing; HE Fang-lian; JIANG Wen; GAO Mei-ping; ZHANG Shang-wen; WANG Yan; WEI Shao-long; JIANG Hui-ping; CHEN Li-juan; BI Zhi-qiang; OU Kun-peng; YAN Mei-xin
- Abstract
【 Objective 】 The present study was conducted to identify the causal pathogen of taro leaf blight disease by using morphology and molecular methods , and establish a protoplast preparation protocol , in order to provide scientific basis for pathogen detection of taro leaf blight , pathogenesis and virus-free and healthy seedling production. 【 Method 】 The samples were collected from taro production area in Lipu county , Guangxi. The pathogen was isolated , and identified through morphological examination combined with rDNA-ITS sequence analysis. Protoplast of pathogen was prepared by enzymatic digestion. 【 Result 】 The taro leaf blight pathogen was preliminarily identified as Phytophthora colocasiae based on its morphological characteristics and pathogenicity test. The results of rDNA-ITS sequence analysis indicated that the nucleotide sequence of the isolate was 99% similar to the isolates of P. colocasiae strains available in GenBank, which confirmed further that the isolate was P. colocasiae. Furthermore, the prepared protoplasts of P. colocasiae were circular or nearly circular, small or large, transparent. 【 Conclusion 】The casual pathogen of taro leaf blight in Lipu of Guangxi is identified as P. colocasiae and its prepared protoplast could be used for pathogenisis study of taro leaf blight disease.
- Subjects
TARO diseases &; pests; BLIGHT diseases (Botany); PLANT protoplasts; PHYTOPATHOGENIC microorganisms; PLANT morphology; NUCLEOTIDE sequence
- Publication
Journal of Southern Agriculture, 2016, Vol 47, Issue 11, p1861
- ISSN
2095-1191
- Publication type
Article
- DOI
10.3969/j:issn.2095-1191.2016.11.1861