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- Title
EFEITOS DO FeCl<sub>2</sub> e CuCl<sub>2</sub> NA ATIVIDADE DA ACETILCOLINESTERASE CEREBRAL DE Oreochromis niloticus.
- Authors
de Melo Oliveira, Vagne; Dias de Assis, Caio Rodrigo; Freitas da Silva, Raquel Pereira; de Souza Bezerra, Ranilson
- Abstract
Acetylcholinesterase (AChE, EC 3.1.1.7) is an enzyme whose classical function is the modulation of nerve impulses in the cholinergic synapses. It is the primary target of some pesticides and its activity can be disturbed by ions. Studies pointed Cu2+ as an AChE inhibitor whereas Fe2+ can inhibit enzymatic activity at higher concentrations. The present work aimed to evaluate in vivo and in vitro the effect of iron chloride (FeCl2) and copper chloride (CuCl2) on the activity of acetylcholinesterase from brain of Nile tilapia, Oreochromis niloticus, fingerlings. The in vivo test with FeCl2 (3 µg/mL) showed a cholinesterase activity of 100.13 ± 24.58%, while in vitro analysis using 0.127; 1.27; 12.7; 126.7 and 1267 µg/mL showed AChE activity of 100.82 ± 5.20%; 101.96 ± 2.45%; 96.27 ± 3.71%; 103.82 ± 1.76% and 89.65 ± 2.43%, respectively, compared to controls. The in vivo test of CuCl2 showed a cholinesterase activity of 88.44 ± 2.01%, whereas in vitro analysis using 0.17; 1.70; 17.0; 170.5 and 1705 µg/mL showed AChE activity of 98.78 ± 5.74%; 81 ± 0.29%; 87.79 ± 10.27%; 81.50 ± 9.84% and 62.55 ± 5.06%, respectively. The results suggest that brain acetylcholinesterase from O. niloticus has low sensitivity to Fe2+ and high sensitivity to Cu2+ only at the concentration of 1705 µg/mL (10 mM), which does not prevent the use of this enzyme as a biomarker of pesticides, since this concentration is only found in nature in samples associated with industrial or mining enterprises.
- Subjects
ACETYLCHOLINESTERASE; IRON chlorides; COPPER chlorides; NILE tilapia; BIOMARKERS; PHYSIOLOGICAL effects of pesticides; FISHES
- Publication
Natural Resources (1984-5901), 2012, Vol 2, Issue 2, p27
- ISSN
1984-5901
- Publication type
Article
- DOI
10.6008/ESS2237-9290.2012.002.0003