We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Presence of Adenovirus-36 DNA in Adipose Tissue of Women: Relationship with Adipocyte Morphology and the Expression of C/EBPβ and HIF-1α.
- Authors
Barrera-Alcocer, Jorge; García-Benavides, Leonel; Muñoz-Valle, José F; de la Cruz-Mosso, Ulises; González, Ramón A; Luquín, Sonia; Alarcón-Romero, Luz del C; Marino-Ortega, Linda A; Matia-Garcia, Ines; Parra-Rojas, Isela
- Abstract
Background: Human adenovirus 36 (HAd36) infection has been associated with obesity. Experiments using 3T3-L1 adipocyte cultured cells and human adipose stem cells (hASCc) have shown that HAd36 stimulates the expression of genes implicated in cell differentiation and increased lipid accumulation. The presence of HAd36 in adipose tissue of overweight and obese women has also been confirmed. This study aims to analyze the presence of HAd36 DNA in the adipose tissue of women undergoing surgery for weight reduction and its relationship with obesity through changes in adipocyte morphology as well as the expression of C/EBPβ and HIF-1α. Methods: Fifty-two subcutaneous adipose tissue biopsies were collected. The anthropometric parameters measured were weight, height, skin folds, body circumferences, and body fat percentage. Biochemical measures were performed for glucose, cholesterol, triglycerides, cholesterol HDL-c, and LDL-c. The presence of HAd36 DNA was performed by conventional PCR. Adipocyte morphology was analyzed in H&E-stained sections using ImageJ/Fiji software. The expression of genes C/EBPβ, HIF-1α and β-actin was determined using TaqMan probes. Results: HAd36 DNA was detected in 31% of adipose tissue samples. The presence of viral DNA was not significantly associated with anthropometric, clinical, or metabolic measurements, or with changes in adipose tissue morphology. The levels of mRNA expression for C/EBPβ and HIF-1α did not show significant differences between positive and negative samples for HAd36 (p> 0.05). Conclusion: The presence of HAd36 DNA in adipose tissue was identified, but it was not related to morphological changes of adipocytes, or the expression of C/EBPβ and HIF-1α. Further studies are needed to confirm these findings.
- Subjects
FIJI; ADIPOSE tissues; FAT; HUMAN stem cells; DNA; WEIGHT loss; MORPHOLOGY
- Publication
Diabetes, Metabolic Syndrome & Obesity: Targets & Therapy, 2021, Vol 14, p477
- ISSN
1178-7007
- Publication type
Article
- DOI
10.2147/DMSO.S285341