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- Title
Dynamic Growth and Shrinkage Govern the pH Dependence of RecA Filament Stability.
- Authors
Kim, Sung Hyun; Park, Jeehae; Joo, Chirlmin; Kim, Doseok; Ha, Taekjip
- Abstract
RecA proteins form a long stable filament on a single-stranded DNA and catalyze strand exchange reaction. The stability of RecA filament changes dramatically with pH, yet its detailed mechanism is not known. Here, using a single molecule assay, we determined the binding and dissociation rates of RecA monomers at the filament ends at various pH. The pH-induced rate changes were moderate but occurred in opposite directions for binding and dissociation, resulting in a substantial increase in filament stability in lower pH. The highly charged residues in C-terminal domain do not contribute to the pH dependent stability. The stability enhancement of RecA filament in low pH may help the cell to cope with acidic stress by fine-tuning of the binding and dissociation rates without losing the highly dynamic nature of the filament required for strand exchange.
- Subjects
PH effect; SINGLE-stranded DNA; EXCHANGE reactions; SINGLE molecules; BIOLOGICAL assay; MONOMERS
- Publication
PLoS ONE, 2015, Vol 10, Issue 1, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0115611