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- Title
Novel Exons and Splice Variants in the Human Antibody Heavy Chain Identified by Single Cell and Single Molecule Sequencing.
- Authors
Vollmers, Christopher; Penland, Lolita; Kanbar, Jad N.; Quake, Stephen R.
- Abstract
Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single molecule sequencing of antibody heavy chain transcripts and were able to identify novel exons for IGHA1 and IGHA2 as well as novel isoforms for IGHM antibody heavy chain.
- Subjects
EXONS (Genetics); GENETIC engineering; SINGLE molecules; NUCLEOTIDE sequencing; GENETIC code
- Publication
PLoS ONE, 2015, Vol 10, Issue 1, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0117050