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- Title
ENHANCEMENT OF CALLUS INDUCTION AND CUCURBITACIN CONTENT IN CITRULLUS COLOCYN THIS L. (SCHRAD) USING PLANT GROWTH REGULATORS.
- Authors
Hegazy, Ahmad K.; Amal A. Mohamed; Ali, Sami l.; Sakere, Mahmoud M.
- Abstract
The objectives of this study were to investigate the effect of growth regulators and different explant type in callus induction and to increase the yield of cucurbitacin in leaf, stem and root explants of Citrullus colocynthis. The specimens were 15 days old and were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of 2, 4- dichlorophenoxy acetic acid (2, 4-D), Kinetin (kin), Benzyl adenine (BA) and a-naphthalene acetic acid (NAA) The different concentrations of 2, 4-D + kin and BA + NAA as well as different explant organ types increased the callus fresh weight and dry weight. The callus cultures derived from stem explants grown on BA + NAA were proved to be an appropriate protocol for callus induction, while 2,4-D failed to stimulate callus growth in the same manner. Different callus explants and the in vitro raised seedling leaf, stem and root were harvested and subjected to extraction of active principle compounds. The results revealed that stem-derived callus cultured on 2,4-D (2 mg/I) + kin (4 mg/I) produced the highest total cucurbitacins content with values reaching 10.89% compared to the control seedling stem, leaf and root which produced (4.95, 4.97 and 5.12%), respectively. The HPLC analysis of cucurbitacin-E showed distinct changes in the different cultures initiated from various explants.
- Subjects
CALLUS; CUCURBITA; PLANT regulators; AGRICULTURAL chemicals; PLANT growth regulation; CITRULLUS; PLANT conservation; REPRODUCTION
- Publication
Journal of the Alabama Academy of Science, 2010, Vol 81, Issue 1, p23
- ISSN
0002-4112
- Publication type
Article