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- Title
Involvement of cardiomyocyte apoptosis in myocardial injury of hereditary epileptic rats.
- Authors
Chen, Fan; Cao, Yong-gang; Qi, Han-ping; Li, Lei; Huang, Wei; Wang, Ye; Sun, Hong-li
- Abstract
Many clinical cases have been reported where epilepsy profoundly influenced the pathophysiological function of the heart; however, the underlying mechanisms were not elucidated. We use the tremor (TRM) rat as an animal model of epilepsy to investigate the potential mechanisms of myocardial injury. Cardiac functions were assessed by arrhythmia score, heart rate, heart:body mass ratio, and hemodynamic parameters including left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and maximum rate of left ventricular pressure rise and fall (+d p/d tmax and -d p/d tmax). Catecholamine level was detected by HPLC. Apoptotic index was estimated by TUNEL assay. The expressions of Bcl-2, Bax, caspase-3, extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal protein kinases (JNK), and p38 were evaluated by Western blot. The results indicated that there existed cardiac dysfunction and cardiomyocyte apoptosis, accompanied by increasing catecholamine levels in TRM rats. Further investigation revealed that apoptosis was mediated by reducing Bcl-2, upregulating Bax, and activating caspase-3. Additional experiments demonstrated that P-ERK1/2 was decreased, whereas P-JNK and P-p38 were up-regulated. Our results suggest that the sympathetic nervous system activation and cardiomyocyte apoptosis are involved in the myocardial injury of TRM rats. The mechanisms of apoptosis might be associated with the activation of the mitochondria-initiated and the mitogen-activated protein kinase pathways.
- Subjects
HEART cells; APOPTOSIS; MYOCARDIAL injury; GENETIC disorders; EPILEPSY; LABORATORY rats
- Publication
Canadian Journal of Physiology & Pharmacology, 2013, Vol 91, Issue 10, p804
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/cjpp-2013-0005