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- Title
C-Kit<sup>+</sup> Cells Isolated from Developing Kidneys Are a Novel Population of Stem Cells with Regenerative Potential.
- Authors
Rangel, Erika B.; Gomes, Samirah A.; Dulce, Raul A.; Premer, Courtney; Rodrigues, Claudia O.; Kanashiro-Takeuchi, Rosemeire M.; Oskouei, Behzad; Carvalho, Decio A.; Ruiz, Phillip; Reiser, Jochen; Hare, Joshua M.
- Abstract
The presence of tissue specific precursor cells is an emerging concept in organ formation and tissue homeostasis. Several progenitors are described in the kidneys. However, their identity as a true stem cell remains elusive. Here, we identify a neonatal kidney-derived c-kit+ cell population that fulfills all of the criteria as a stem cell. These cells were found in the thick ascending limb of Henle's loop and exhibited clonogenicity, self-renewal, and multipotentiality with differentiation capacity into mesoderm and ectoderm progeny. Additionally, c-kit+ cells formed spheres in nonadherent conditions when plated at clonal density and expressed markers of stem cells, progenitors, and differentiated cells. Ex vivo expanded c-kit+ cells integrated into several compartments of the kidney, including tubules, vessels, and glomeruli, and contributed to functional and morphological improvement of the kidney following acute ischemia-reperfusion injury in rats. Together, these findings document a novel neonatal rat kidney c-kit+ stem cell population that can be isolated, expanded, cloned, differentiated, and used for kidney repair following acute kidney injury. These cells have important biological and therapeutic implications. S TEM C ells 2013;31:1644-1656
- Subjects
CYTOLOGICAL research; HOMEOSTASIS; STEM cells; KIDNEY diseases; KIDNEY tubules; CELL populations; THERAPEUTICS
- Publication
Stem Cells, 2013, Vol 31, Issue 8, p1644
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1002/stem.1412