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- Title
Interaction between β-Adrenergic Receptor Stimulation and Nitric Oxide Release on Tissue Perfusion and Metabolism.
- Authors
JORDAN, JENS; TANK, JENS; STOFFELS, MANDY; FRANKE, GABRIELE; CHRISTENSEN, NIELS JUEL; LUFT, FRIEDRICH C.; BOSCHMANN, MICHAEL
- Abstract
Nitric oxide (NO) may be an important modulator of sympathetic tone. We used im and sc microdialysis in humans to characterize the interaction of NO synthase inhibition and adrenoreceptor stimulation on tissue perfusion, metabolism, and norepinephrine release. Microdialysis probes were perfused with L- or D-nitro-L-arginine-methyl-ester (100 μmol/L) followed by incremental doses of isoproterenol, epinephrine, or nitroprusside. Blood flow was estimated based on the ethanol dilution technique. In muscle, the increase in blood flow with isoproterenol was abolished by L-NAME. The ethanol ratio was 0.03 ± 0.011 with D-NAME and 0.075 ± 0.014 with L-NAME during isoproterenol treatment (1μ mol/L). The effect was less pronounced in adipose tissue. The vasodilatory effect of nitroprusside was similar with D- and L-NAME. L-NAME augmented isoproterenol- and epinephrine-induced glycerol release. Dialysate glycerol during 1 μmol/L isoproterenol was 47 ± 6.7 μmol/L with D-NAME and 72 ± 15 μmol/L with L-NAME. In skeletal muscle, dialysate norepinephrine during 1 μmol/L isoproterenol treatment was 0.73 ± 0.17 and 1.3± 0.15 nmol/L with D- and L-NAME, respectively. We conclude that NO synthase inhibition attenuatesβ 2-adrenoreceptor-mediated vasodilation and enhancesβ -adrenoreceptor-mediated lipolysis. These effects are in part mediated through an increase in interstitial norepinephrine concentrations. The data are consistent with the idea that in humans, NO is important in modulating and ameliorating sympathetic effects in peripheral tissues.
- Publication
Journal of Clinical Endocrinology & Metabolism, 2001, Vol 86, Issue 6, p2803
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/jcem.86.6.7567