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- Title
Brain death induces renal expression of heme oxygenase-1 and heat shock protein 70.
- Authors
van Dullemen, Leon F. A.; Bos, Eelke M.; Schuurs, Theo A.; Kampinga, Harm H.; Ploeg, Rutger J.; van Goor, Harry; Leuvenink, Henri G. D.
- Abstract
Background: Kidneys derived from brain dead donors have lower graft survival and higher graft-function loss compared to their living donor counterpart. Heat Shock Proteins (HSP) are a large family of stress proteins involved in maintaining cell homeostasis. We studied the role of stress-inducible genes Heme Oxygenase-1 (HO-1), HSP27, HSP40, and HSP70 in the kidney following a 4 hour period of brain death. Methods: Brain death was induced in rats (n=6) by inflating a balloon catheter in the epidural space. Kidneys were analysed for HSPs using RT-PCR, Western blotting, and immunohistochemistry. Results: RT-PCR data showed a significant increase in gene expression for HO-1 and HSP70 in kidneys of brain dead rats. Western blotting revealed a massive increase in HO-1 protein in brain dead rat kidneys. Immunohistochemistry confirmed these findings, showing extensive HO-1 protein expression in the renal cortical tubules of brain dead rats. HSP70 protein was predominantly increased in renal distal tubules of brain dead rats treated for hypotension. Conclusion: Renal stress caused by brain death induces expression of the cytoprotective genes HO-1 and HSP70, but not of HSP27 and HSP40. The upregulation of these cytoprotective genes indicate that renal damage occurs during brain death, and could be part of a protective or recuperative mechanism induced by brain death-associated stress.
- Subjects
BRAIN death; OXYGENASES; HEAT shock proteins; KIDNEYS; LABORATORY rats; IMMUNOHISTOCHEMISTRY; WESTERN immunoblotting
- Publication
Journal of Translational Medicine, 2013, Vol 11, Issue 1, p1
- ISSN
1479-5876
- Publication type
Article
- DOI
10.1186/1479-5876-11-22