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- Title
The rapid emergence of KPC-2-producing Klebsiella pneumoniae ST307 in general hospitals in Gyeongsang-do, Republic of Korea.
- Authors
Min-Kyeong Kim; Jungsun Park; Seong-je Joo; Eunkyung Shin; Hyun Ju Jeong; Jaeil Yoo; Junyoung Kim
- Abstract
배경 In healthcare infections, Carbapenem-resistant Klebsiella pneumoniae (CRKP), as multi-drug resistant bacteria, constitutes a serious threat to public health. The occurrence of CRKP infection cases is highly concentrated in Gyeongsang-do. In this study, we investigated the molecular characteristics of CRKP collected from Gyeongsang-do. 방법 Based on identified Klebsiella pneumoniae carbapenemase (KPC) type and the antimicrobial resistance profile (MICs > 16 mg/L), 44 CRKP isolates from 19 hospitals were subjected to XbaI-pulsed field gel electrophoresis (PFGE), and whole-genome sequencing (WGS) by Miseq platform. The molecular genetic analysis was determined by CLC genome workbench and Center for genomic epidemiology services. 결과 All CRKP isolates exhibited multidrug resistance to carbapenems, cephems and fluoroquinolones, harboring a blaKPC-2 gene. Twenty-four unique XbaI-PFGE patterns were identified and tightly clustered on similarity value of ≥ 90 %. In whole genome analysis, isolates were identified as multilocus sequence typing (MLST) sequence type 307, resistance gene profiles (blaKPC-2, blaCTX-M-15, blaOXA-1, blaTEM-1B, aac (6′)-Ib-cr, oqxAB, catB3, qnrB1, dfrA14, and sul2) and plasmid replicons (IncFIB(K), IncFII(K), IncX3) were detected. The genetic relationships showed that the KPC-2-producing CRKP ST307 highly related by whole genome multilocus sequence typing (wgMLST). 결론 The finding suggested that laboratory surveillance is necessary to control the dissemination of CRKP. Therefore, more comprehensive surveillance is required to strengthen monitoring for prevent the spread of high-risk clone.
- Subjects
SOUTH Korea; KLEBSIELLA pneumoniae; MULTIDRUG resistance in bacteria; CARBAPENEM-resistant bacteria; WHOLE genome sequencing; NUCLEOTIDE sequencing; MULTIDRUG resistance
- Publication
Infection & Chemotherapy, 2022, Vol 54, pS269
- ISSN
2093-2340
- Publication type
Article