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- Title
Molecular cloning, expression and characterization of a novel feruloyl esterase from a soil metagenomic library with phthalate-degrading activity.
- Authors
Wu, Shenglu; Nan, Fang; Jiang, Junwei; Qiu, Jiarong; Zhang, Yueqi; Qiao, Beibei; Li, Shan; Xin, Zhihong
- Abstract
Objectives: To discover novel feruloyl esterases (FAEs) by the function-driven screening procedure from soil metagenome. Results: A novel FAE gene bds4 was isolated from a soil metagenomic library and over-expressed in Escherichia coli. The recombinant enzyme BDS4 was purified to homogeneity with a predicted molecular weight of 38.8 kDa. BDS4 exhibited strong activity (57.05 U/mg) toward methyl ferulate under the optimum pH and temperature of 8.0 and 37°C. Based on its amino acid sequence and model substrates specificity, BDS4 was classified as a type-C FAE. The quantity of the releasing ferulic acid can be enhanced significantly in the presence of xylanase compared with BDS4 alone from de-starched wheat bran. In addition, BDS4 can also hydrolyze several phthalates such as diethyl phthalate, dimethyl phthalate and dibutyl phthalate. Conclusion: The current investigation discovered a novel FAE with phthalate-degrading activity and highlighted the usefulness of metagenomic approaches as a powerful tool for discovery of novel FAEs.
- Subjects
FERULIC acid; MOLECULAR cloning; DIETHYL phthalate; DIBUTYL phthalate; AMINO acid sequence; WHEAT bran
- Publication
Biotechnology Letters, 2019, Vol 41, Issue 8/9, p995
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-019-02693-3