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- Title
Production of 5,8-dihydroxy-9( Z)-octadecenoic acid from oleic acid by whole recombinant cells of Aspergillus nidulans expressing diol synthase.
- Authors
Seo, Min-Ju; Shin, Kyung-Chul; Jeong, Yeon-Ju; Oh, Deok-Kun
- Abstract
The optimal conditions for the production of 5,8-dihydroxy-9( Z)-octadecenoic acid (5,8-diHOME) from oleic acid by whole recombinant Escherichia coli cells expressing diol synthase from Aspergillus nidulans were 40 °C, pH 7.5, 10 % (v/v) dimethyl sulfoxide, 35 g cells l, and 12 g oleic acid l at 250 rpm in a 250 ml baffled flask. Under these conditions, whole recombinant cells produced 5.2 g 5,8-diHOME l together with 1 g l of the intermediate 8-hydroperoxy-9( Z)-octadecenoic acid (8-HPOME) after 60 min. This corresponded to a conversion yield of 43 % (w/w), a volumetric productivity of 5.2 g lh, and a specific productivity of 148 mg g cellsh. This is the first report of the biotechnological production of 5,8-diHOME from oleic acid.
- Subjects
OLEIC acid; ASPERGILLUS nidulans; SYNTHASES; ESCHERICHIA coli; GENE expression in bacteria; BIOTECHNOLOGY
- Publication
Biotechnology Letters, 2015, Vol 37, Issue 1, p131
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1650-y