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- Title
Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum.
- Authors
Kang, Min-Kyoung; Eom, Jin-Hee; Kim, Yunje; Um, Youngsoon; Woo, Han
- Abstract
Pinene is a monoterpenes (C) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS ( P. taeda) and GPPS ( A. grandis) resulted in 27 μg ± 7 α-pinene g cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production.
- Subjects
BIOSYNTHESIS; PINENE; GLUCOSE; CORYNEBACTERIUM glutamicum; GENETIC engineering; BIOMASS energy; ISOMERASES
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 10, p2069
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1578-2