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- Title
Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease.
- Authors
Youm, Jung; Jeon, Jae; Kim, Hee; Kim, Young; Ko, Kisung; Joung, Hyouk; Kim, HyunSoon
- Abstract
Human β-amyloid (Aβ) is believed to be one of the main components of Alzheimer’s disease, so reduction of Aβ is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Aβ with tandem repeats. Integration of the human Aβ gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the Aβ protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human Aβ antigen.
- Subjects
TOMATOES; TRANSGENIC plants; AMYLOID beta-protein; VACCINES; ALZHEIMER'S disease; AGROBACTERIUM
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 10, p1839
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-008-9759-5