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- Title
Pilot-Scale Fermentation and Purification of the Recombinant Allophycocyanin Over-Expressed in Escherichia coli.
- Authors
Baosheng Ge; Zhihong Tang; Liping Lin; Yuhong Ren; Yu Yang; Song Qin
- Abstract
A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg−1 dry cells. Recombinant allophycocyanin significantly inhibited H22 hepatoma ( p ( 0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg−1 d−1.
- Subjects
ANTINEOPLASTIC agents; ESCHERICHIA coli; RECOMBINANT proteins; PROTEINS; CHROMATOGRAPHIC analysis
- Publication
Biotechnology Letters, 2005, Vol 27, Issue 11, p783
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-005-5794-7