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- Title
UT-B Urea Transporter Localization in the Bovine Gastrointestinal Tract.
- Authors
Coyle, J.; McDaid, S.; Walpole, C.; Stewart, Gavin; Stewart, Gavin S
- Abstract
Facilitative UT-B urea transporters play an important role in the urea nitrogen salvaging process that occurs in the gastrointestinal tract of mammals, particularly ruminants. Gastrointestinal UT-B transporters have previously been reported in various ruminant species-including cow, sheep and goat. In this present study, UT-B transporter localization was investigated in tissues throughout the bovine gastrointestinal tract. RT-PCR analysis showed that UT-B2 was the predominant UT-B mRNA transcript expressed in dorsal, ventral and cranial ruminal sacs, while alternative UT-B transcripts were present in other gastrointestinal tissues. Immunoblotting analysis detected a strong, glycosylated ~50 kDa UT-B2 protein in all three ruminal sacs. Immunolocalization studies showed that UT-B2 protein was predominantly localized to the plasma membrane of cells in the stratum basale layer of all ruminal sac papillae. In contrast, other UT-B protein staining was detected in the basolateral membranes of the surface epithelial cells lining the abomasum, colon and rectum. Overall, these findings confirm that UT-B2 cellular localization is similar in all ruminal sacs and that other UT-B proteins are located in epithelial cells lining various tissues in the bovine gastrointestinal tract.
- Subjects
GASTROINTESTINAL system; BOVINE anatomy; UREA transporters; REVERSE transcriptase polymerase chain reaction; RUMEN (Ruminants); CELL membranes; RNA metabolism; ANIMAL experimentation; CATTLE; GENE expression; IMMUNOHISTOCHEMISTRY; RNA; STOMACH; MEMBRANE transport proteins
- Publication
Journal of Membrane Biology, 2016, Vol 249, Issue 1/2, p77
- ISSN
0022-2631
- Publication type
journal article
- DOI
10.1007/s00232-015-9850-5