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- Title
Developmental competence of mouse embryos following zona drilling using a non-contact holmium:yttrium scandian gallium garnet (Ho:YSGG) laser system.
- Authors
Schiewe, Mitchel C.; Neev, Joseph; Hazeleger, Nancy L.; Balmaceda, Jose P.; Berns, Michael W.; Tadir, Yona; Schiewe, M C; Neev, J; Hazeleger, N L; Balmaceda, J P; Berns, M W; Tadir, Y
- Abstract
The purpose of this study was to assess the efficacy of the holmium: yttrium scandian gallium garnet (Ho:YSGG) laser, operating in a pipette-free, non-contact mode, to assist hatching and sustain normal embryonic development. Two-cell mouse embryos were recovered and assigned to laser-assisted hatching (LAH) treatment or control human tubal fluid (HTF) culture with or without serum (HTF-s, HTF-o) or with late serum supplementation (HTF-o/s). The basic experimental apparatus for LAH consisted of a stationary 2.1 μm Ho: YSGG laser beam directed through a mechanical shutter into an input port of a Zeiss Axiomat inverted microscope. Fewer (P < 0.05) embryos developed to the blastocyst stage in the HTF-s group (81%) than in the LAH (90%), HTF-o (94%) and HTF-o/s (92%) groups. The level of hatching was significantly increased ( < 0.01) after the LAH treatment (57%) compared to HTF-o/s (32%), HTF-s (18%) or HTF-o (5%). Implantation rates were not significantly impaired following the LAH treatment (21%). These data demonstrate that LAH using the Ho: YSGG laser is a simple, accurate and effective procedure for assisted hatching.
- Publication
Human Reproduction, 1995, Vol 10, Issue 7, p1821
- ISSN
0268-1161
- Publication type
journal article