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- Title
Amplification of X- and Y-chromosome-specific regions from single human blastomeres by polymerase chain reaction for sexing of preimplantation embryos.
- Authors
Liu, Jiaen; Lissens, Willy; Devroey, Paul; Van Steirteghem, Andre; Liebaers, Ingeborg; Liu, J; Lissens, W; Devroey, P; Van Steirteghem, A; Liebaers, I
- Abstract
We describe a polymerase chain reaction (PCR) assay for the simultaneous detection of X and Y chromosomes in human blastomeres. In a first round of PCR, both X- and Y-specific fragments are amplified with primers which are common to both chromosomes and are derived from the X-linked steroid sulphatase gene and the Y-linked pseudogene. In a second round of PCR, fragments specific to each chromosome are generated: both an X and a Y fragment in male embryos and, due to the absence of a Y chromosome, only an X fragment in females. The efficiency and accuracy of this assay are high; it generates no false positive amplification signals and allows sexing in approximately 6 h after embryo biopsy. We therefore believe that it is suitable for gender determination by preimplantation diagnosis for couples at risk for X-linked genetic diseases.
- Publication
Human Reproduction, 1994, Vol 9, Issue 4, p716
- ISSN
0268-1161
- Publication type
journal article