We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Alpha Amylase from Bacillus pacificus Associated with Brown Algae Turbinaria ornata : Cultural Conditions, Purification, and Biochemical Characterization.
- Authors
Alonazi, Mona; Karray, Aida; Badjah-Hadj-Ahmed, Ahmed Yacine; Ben Bacha, Abir
- Abstract
We aimed in the current study, the identification of a marine bacterial amylase produced by Bacillus pacificus, which was associated with Turbinaria ornata. Cultural conditions were optimized for the highest amylase production on Tryptic soy broth media supplemented with starch 1% at initial pH 9, 55 °C for 24 h. The newly purified amylase was characterized for a possible biotechnological application. Data indicated that the obtained amylase with a molecular weight of 40 kD and the N-terminal sequence of the first 30 amino acids of amBp showed a high degree of homology with known alpha amylase, and was stable at 60 °C of pH 11. Among the tested substrate analogs, amBp was almost fully active on Alylose and Alylopectine (97%), but moderately hydrolyzed glycogen < sucrose < maltose < lactose. Therefore, the current amylase mainly generated maltohexaose from starch. Mg2+ and Zn2+ improved amylase activity up to 170%. While ethylenediamine tetraacetic acid (EDTA) similarly induced the greatest activity with purified amylase, PCMB had the least effect. Regarding all these characteristics, amylase from marine bacterial symbionts amBp has a new promising feature for probable therapeutic, industrial, and nutritional applications.
- Subjects
BROWN algae; AMYLASES; MOLECULAR weights; ETHYLENEDIAMINE; MALTOSE; AMINO acids
- Publication
Processes, 2021, Vol 9, Issue 1, p16
- ISSN
2227-9717
- Publication type
Article
- DOI
10.3390/pr9010016