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- Title
A Cyanophage MarR-Type Transcription Factor Regulates Host RNase E Expression during Infection.
- Authors
Lambrecht, S. Joke; Stappert, Nils; Sommer, Frederik; Schroda, Michael; Steglich, Claudia
- Abstract
The marine picocyanobacterium Prochlorococcus contributes significantly to global primary production, and its abundance and diversity is shaped in part by viral infection. Here, we identified a cyanophage-encoded MarR-type transcription factor that induces the gene expression of host Prochlorococcus MED4 endoribonuclease (RNase) E during phage infection. The increase in rne transcript levels relies on the phage (p)MarR-mediated activation of an alternative promoter that gives rise to a truncated yet enzymatically fully functional RNase E isoform. In this study, we demonstrate that pMarR binds to an atypical activator site downstream of the transcriptional start site and that binding is enhanced in the presence of Ca2+ ions. Furthermore, we show that dimeric pMarR interacts with the α subunit of RNA polymerase, and we identified amino acid residues S66, R67, and G106, which are important for Ca2+ binding, DNA binding, and dimerization of pMarR, respectively.
- Subjects
GENE expression; AMINO acid residues; RNA polymerases; PROCHLOROCOCCUS; VIRUS diseases; TRANSCRIPTION factors
- Publication
Microorganisms, 2022, Vol 10, Issue 11, p2245
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms10112245