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- Title
The use of flow cytometry for the detection of CMV-specific antigen (pp65) in leukocytes of kidney recipients.
- Authors
Essa, Sahar; Pacsa, Alexander S; Al-Attiyah, Raja'a; El-Shazly, Amany; Raghupathy, Raj; Said, Tareq
- Abstract
Flow cytometric assay (FCA) was used to detect a cytomegalovirus (CMV) specific antigen (pp65) in CMV-infected fibroblast cells and in leukocytes of kidney recipients. FCA distinguished clearly between the infected and non-infected fibroblast cells. Regarding transplant patients, the FCA was positive when the number of antigenemia assay (AA) positive cells was five or more per 5×104. Moreover, the percentage of antigenemia-positive cells by FCA correlated well with symptomatic CMV infections.
- Subjects
FLOW cytometry; CYTOMEGALOVIRUSES; ANTIGENS; KIDNEY transplant patients; LEUCOCYTES
- Publication
Clinical Transplantation, 2000, Vol 14, Issue 2, p147
- ISSN
0902-0063
- Publication type
Article
- DOI
10.1034/j.1399-0012.2000.140208.x