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- Title
Enhancement of nisin production in milk by conjugal transfer of the protease-lactose plasmid pLP712 to the wild strain Lactococcus lactis UQ2.
- Authors
García-Parra, MaríA D; Campelo, Ana B.; García-Almendárez, Blanca E.; Regalado, Carlos; Rodríguez, Ana; Martínez, Beatriz
- Abstract
Lactococcus lactis UQ2 is a wild nisin A producer isolated from a Mexican cheese that grows poorly in milk. Conjugal matings with L. lactis NCDO712 to transfer the Lac+ Prt+ plasmid pLP712 and selection with nisin and lactose yielded L. lactis NCDO712 NisA+. Naturally rifampicin resistant L. lactis UQ2Rif was isolated to provide an additional selective marker. The identity of a transconjugant L. lactis UQ2Rif Lac+ was confirmed by RAPD-PCR fingerprinting, nisA PCR amplification, nisin production, presence of pLP712 and phospho-β-galactosidase activity. This strain performed well in milk and synthesised 200 IU/mL nisin, 40 times more than the original strain.
- Subjects
LACTOCOCCUS lactis; MILK microbiology; PROTEOLYTIC enzymes; LACTOSE; NISIN; PLASMIDS; CHEESE microbiology; RIFAMPIN
- Publication
International Journal of Dairy Technology, 2010, Vol 63, Issue 4, p523
- ISSN
1364-727X
- Publication type
Article
- DOI
10.1111/j.1471-0307.2010.00618.x