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- Title
Synthesis, spectroscopic, characterization, pharmacological evaluation, and cytotoxicity assays of novel nano and micro scale of copper (II) complexes against human breast cancer cells.
- Authors
Ali, Atheer A.; Al-Hassani, Rehab M.; Hussain, Dhia H.; Rheima, Ahmed Mahdi; Meteab, Hamid S.
- Abstract
Introduction: Although 1,2,4-triazoles-3-thione derivatives have an important biological active, azomethine group in Schiff base triazole are to be bioactive compounds and its complexes having metals, copper has been a subject of interest; it is bound with ligands creating complexes that interact with biomolecules. The use of nano scale from organic compounds has several benefits such as high surface/volume ratios, it shows a considerable biochemical certificate. Objectives: The primary objective of this study was to describe cytotoxicity assays of ligand of novel nano and micro scale of copper (II) complexes against human breast cancer cells. Methods: In this paper synthesized Schiff base triazole derivative (L1) that containing 1,2,4-triazole ring by condensation of 4-hydroxybenzaldehyd with 4'-amino-5-(naphthalen-1-ylmethyl)-4H-1,2,4- triazole-3-thiol then characterized ligand (L1) by elemental analyses (C.H.N.S), nuclear magnetic resonance (¹HNMR and 13CNMR) spectra, Fourier-transform infrared (FTIR), ultraviolet visible (UV-vis) spectroscopic and mass spectrum. The new asymmetrical Schiff base (L1) used as a ligand to synthesize novel nano and micro complexes with CuCl2.2H2O. Then was characterized structure of the micro complex by elemental analysis and flame atomic absorption in addition to Fouriertransform infrared and ultraviolet visible spectra. The magnetic susceptibility properties and conduct metric measurements of the metal complex were also determined; the structure of nano complex characterized using Fourier-transform infrared, atomic force microscope (AFM) and scanning electron microscope (SEM). Evaluated antioxidant activity (DPPH Assay) by Examine novel compounds for the DPPH scavenging activities and tested novel compounds by MTT Assay, this method using 96-well microtiter plates containing RPMI medium for seeding of the cells (1× 105 cells/mL). Results: The antioxidant and cytotoxic activities for the ligand (L1) and complexes (nano, micro) were studied in the inhibition of human breast cancer cells MCF-7 ligand (L1) < micro complexes < nano complexes. Conclusions: The results exhibit that the Schiff base triazole ligand (L1) and nano ligand (L1 nano) act as a bidentate chelating agent, bonded to the metal ion through the N atom of the azomethine and S of thiocarbonyl groups. These compounds presented potent anti-proliferative activity against MCF-7 cells at low concentrations. Our result suggested that a novel synthetic compound may be apotential breast cancer inhibitor.
- Subjects
CANCER cells; BREAST cancer; SCHIFF base derivatives; ATOMIC force microscopes; COMPLEX compounds; SCHIFF bases
- Publication
Drug Invention Today, 2020, Vol 14, Issue 1, p31
- ISSN
0975-7619
- Publication type
Article