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- Title
Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3.
- Authors
Jiwen Li; Jin Wang; Jianxiang Wang; Nawaz, Zafar; Liu, Johnson M.; Jun Qin; Jiemin Wong
- Abstract
We present evidence that both corepressors SMRT and N-CoR exist in large protein complexes with estimated sizes of 1.5–2 MDa in HeLa nuclear extracts. Using a combination of conventional and immunoaffinity chromatography, we have successfully isolated a SMRT complex and identified histone deacetylase 3 (HDAC3) and transducin (Β)-like I (TBL1), a WD-40 repeat-containing protein, as the subunits of the purified SMRT complex. We show that the HDAC3-Containing SMRT and N-CoR complexes can bind to unliganded thyroid hormone receptors (TRs) in vitro. We demonstrate further that in Xenopus oocytes, both SMRT and N-CoR also associate with HDAC3 in large protein complexes and that injection of antibodies against HDAC3 or SMRT/N-CoR led to a partial relief of repression by unliganded TR/RXR. These findings thus establish both SMRT and N-CoR complexes as bona fide HDAC-containing complexes and shed new light on the molecular pathways by which N-CoR and SMRT function in transcriptional repression.
- Subjects
HISTONE deacetylase; AMIDASES; HYDROLASES; PROTEINS; MOLECULAR biology; BIOCHEMISTRY
- Publication
EMBO Journal, 2000, Vol 19, Issue 16, p4342
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/19.16.4342