We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effect of fibroblast growth factor 9 on the osteogenic differentiation of bone marrow stromal stem cells and dental pulp stem cells.
- Authors
JINGTING LU; JIEWEN DAI; XUDONG WANG; MAOLIN ZHANG; PENG ZHANG; HAO SUN; XIULI ZHANG; HONGBO YU; WENBIN ZHANG; LEI ZHANG; XINQUAN JIANG; GUOFANG SHEN, STEVE
- Abstract
The role of fibroblast growth factor 9 (FGF9) in bone formation may depend on gene dosage, developmental stage, cell type or interactions with other cytokines. In the present study bone marrow stromal stem cells (BMSCs) and dental pulp stem cells (DPSCs) were cultured and osteogenically induced in vitro, treated with exogenous FGF9 at varying concentrations. Alkaline phosphatase staining, alizarin red S staining, reverse transcription quantitative polymerase chain reaction and western blot analyses were performed in order to investigate the gene expression levels of osteogenic markers. The results of the present study demonstrated that FGF9 enhanced the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) during osteogenic induction in BMSCs and DPSCs, which are derived from different tissues. FGF9 also inhibited the osteogenic differentiation of BMSCs and DPSCs through the activation of ERK1/2. These findings suggested that FGF9 may be an inhibitor of osteogenesis in mesenchymal stem cells in vitro and its application in vivo requires investigation in the future.
- Subjects
FIBROBLAST growth factors; MESENCHYMAL stem cells; DENTAL pulp; CYTOKINES; ALKALINE phosphatase; GENE expression
- Publication
Molecular Medicine Reports, 2015, Vol 11, Issue 3, p1661
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2014.2998