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- Title
Immunoinformatics-Aided Design of a Peptide Based Multiepitope Vaccine Targeting Glycoproteins and Membrane Proteins against Monkeypox Virus.
- Authors
Akhtar, Nahid; Kaushik, Vikas; Grewal, Ravneet Kaur; Wani, Atif Khurshid; Suwattanasophon, Chonticha; Choowongkomon, Kiattawee; Oliva, Romina; Shaikh, Abdul Rajjak; Cavallo, Luigi; Chawla, Mohit
- Abstract
Monkeypox is a self-limiting zoonotic viral disease and causes smallpox-like symptoms. The disease has a case fatality ratio of 3–6% and, recently, a multi-country outbreak of the disease has occurred. The currently available vaccines that have provided immunization against monkeypox are classified as live attenuated vaccinia virus-based vaccines, which pose challenges of safety and efficacy in chronic infections. In this study, we have used an immunoinformatics-aided design of a multi-epitope vaccine (MEV) candidate by targeting monkeypox virus (MPXV) glycoproteins and membrane proteins. From these proteins, seven epitopes (two T-helper cell epitopes, four T-cytotoxic cell epitopes and one linear B cell epitopes) were finally selected and predicted as antigenic, non-allergic, interferon-γ activating and non-toxic. These epitopes were linked to adjuvants to design a non-allergic and antigenic candidate MPXV-MEV. Further, molecular docking and molecular dynamics simulations predicted stable interactions between predicted MEV and human receptor TLR5. Finally, the immune-simulation analysis showed that the candidate MPXV-MEV could elicit a human immune response. The results obtained from these in silico experiments are promising but require further validation through additional in vivo experiments.
- Subjects
MEMBRANE glycoproteins; MONKEYPOX; MEMBRANE proteins; PEPTIDES; MOLECULAR dynamics
- Publication
Viruses (1999-4915), 2022, Vol 14, Issue 11, p2374
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v14112374