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- Title
A Linear Surface Epitope in a Proline-Rich Region of ORF3 Product of Genotype 1 Hepatitis E Virus.
- Authors
Yonglin Yang; Shaoli Lin; Yuchen Nan; Zexu Ma; Liping Yang; Yanjin Zhang
- Abstract
Hepatitis E virus (HEV) is one of the viral pathogens causing hepatitis in humans. HEV open reading frame 3 (ORF3) encodes a small multifunctional protein (VP13), which is essential for HEV infection. In this study, a linear epitope was identified in a polyproline (PXXP) motif from VP13 of genotype 1 HEV by using amonoclonal antibody. The epitope was detected in enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence assays. Epitope mapping showed that the epitope locates in a proline-rich region containing a PXXP motif in amino acid residues 66-75 of VP13. The epitope was also detected in HEV-infected liver cells and reacted with genotype 1-specific antibodies in an HEV-positive human serum sample. The results demonstrated that the epitope in the PXXP motif of the genotype 1 VP13 is linear and surface-oriented, which should facilitate in-depth studies on the viral protein and HEV biology.
- Subjects
HEPATITIS E virus; OPEN reading frames (Genetics); POLYPROLINE; EPITOPES; ENZYME-linked immunosorbent assay; VIRUSES
- Publication
Viruses (1999-4915), 2016, Vol 8, Issue 8, p227
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v8080227