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- Title
Hydroxyl Radical Generation and DNA Nuclease Activity: A Mechanistic Study Based on a Surface-Immobilized Copper Thioether Clip-Phen Derivative.
- Authors
Romo, Adolfo I. B.; Abreu, Dieric S.; de F. Paulo, Tércio; Carepo, Marta S. P.; Sousa, Eduardo H. S.; Lemus, Luis; Aliaga, Carolina; Batista, Alzir A.; Nascimento, Otaciro R.; Abruña, Héctor D.; Diógenes, Izaura C. N.
- Abstract
Coordination compounds of copper have been invoked as major actors in processes involving the reduction of molecular oxygen, mostly with the generation of radical species the assignment for which has, so far, not been fully addressed. In the present work, we have carried out studies in solution and on surfaces to gain insights into the nature of the radical oxygen species (ROS) generated by a copper(II) coordination compound containing a thioether clip-phen derivative, 1,3-bis(1,10-phenanthrolin-2-yloxy)-N-(4-(methylthio)benzylidene)propan-2-amine (2CP-Bz-SMe), enabling its adsorption/immobilization to gold surfaces. Whereas surface plasmon resonance (SPR) and electrochemistry of the adsorbed complex indicated the formation of a dimeric CuI intermediate containing molecular oxygen as a bridging ligand, scanning electrochemical microscopy (SECM) and nuclease assays pointed to the generation of a ROS species. Electron paramagnetic resonance (EPR) data reinforced such conclusions, indicating that radical production was dependent on the amount of oxygen and H2O2, thus pointing to a mechanism involving a Fenton-like reaction that results in the production of OH..
- Subjects
HYDROXYL group; CHEMICAL derivatives; COORDINATION compounds; COPPER; ELECTROCHEMISTRY
- Publication
Chemistry - A European Journal, 2016, Vol 22, Issue 29, p10081
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201601719