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- Title
Diversity of anaerobic arsenite-oxidizing bacteria in low-salt environments analyzed with a newly developed PCR-based method.
- Authors
Ospino, Melody Cabrera; Kojima, Hisaya; Watanabe, Tomohiro; Iwata, Tomoya; Fukui, Manabu
- Abstract
Anaerobic arsenite oxidation is potentially important but the least understood process in the arsenic cycle. The catalytic subunit of the key enzyme for anaerobic arsenite oxidation is encoded by the <italic>arxA</italic> gene. In this study, a novel primer pair for the <italic>arxA</italic> gene was designed to detect diverse sequences of this notable gene. Further modification of the designed primer was made by adding extra bases to its 5′- end. This modification made it possible to analyze the PCR products with TA cloning, which provides higher throughput of investigations. With the combination of modified primer pair and TA cloning, diverse <italic>arxA</italic> gene sequences were effectively obtained from samples of lake water, spring water, and hot spring microbial mat. The sequences detected in the samples characterized by low salinity and nearly neutral pH were phylogenetically distinct from the majority of previously known <italic>arxA</italic> genes, found in the genome of alkaliphiles and halophiles.
- Subjects
ARSENIC cycle (Biogeochemistry); MICROBIAL mats; HALOPHILIC microorganisms; ARSENITES; POLYMERASE chain reaction
- Publication
Limnology, 2018, Vol 19, Issue 2, p177
- ISSN
1439-8621
- Publication type
Article
- DOI
10.1007/s10201-018-0539-1