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- Title
Gene transcription profiling of Fusarium graminearum treated with an azole fungicide tebuconazole.
- Authors
Xin Liu; Jinhua Jiang; Jiaofang Shao; Yanni Yin; Zhonghua Ma
- Abstract
Using a deep serial analysis of gene expression (DeepSAGE) sequencing approach, we profiled the transcriptional response of Fusarium graminearum to tebuconazole, a most widely used azole fungicide. By comparing the expression of genes in F. graminearum treated and untreated with tebuconazole, we identified 324 and 155 genes showing more than a 5-fold increase and decrease, respectively, in expression upon tebuconazole treatment. These genes are involved in a variety of cell functions including egrosterol biosynthesis, transcription, and cellular metabolism. The validity of DeepSAGE results were confirmed by real-time PCR analysis of expression of 20 genes with different expression levels in the DeepSAGE analysis. The results from this study provide useful information in understanding the mechanisms for the responses of F. graminearum to azole fungicides.
- Subjects
WOOD preservatives; FUSARIUM oxysporum; BIOCHEMISTRY; GENETIC regulation; AGRICULTURAL chemicals; GENE expression; BIOCHEMICAL engineering; BIOCHEMICAL templates; TREE diseases &; pests
- Publication
Applied Microbiology & Biotechnology, 2010, Vol 85, Issue 4, p1105
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-009-2273-4