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- Title
Efficacy of rhBMP-2 Loaded PCL/β-TCP/bdECM Scaffold Fabricated by 3D Printing Technology on Bone Regeneration.
- Authors
Bae, Eun-Bin; Park, Keun-Ho; Shim, Jin-Hyung; Chung, Ho-Yun; Choi, Jae-Won; Lee, Jin-Ju; Kim, Chang-Hwan; Jeon, Ho-Jun; Kang, Seong-Soo; Huh, Jung-Bo
- Abstract
This study was undertaken to evaluate the effect of 3D printed polycaprolactone (PCL)/<italic>β</italic>-tricalcium phosphate (<italic>β</italic>-TCP) scaffold containing bone demineralized and decellularized extracellular matrix (bdECM) and human recombinant bone morphogenetic protein-2 (rhBMP-2) on bone regeneration. Scaffolds were divided into PCL/<italic>β</italic>-TCP, PCL/<italic>β</italic>-TCP/bdECM, and PCL/<italic>β</italic>-TCP/bdECM/BMP groups. In vitro release kinetics of rhBMP-2 were determined with respect to cell proliferation and osteogenic differentiation. These three reconstructive materials were implanted into 8 mm diameter calvarial bone defect in male Sprague-Dawley rats. Animals were sacrificed four weeks after implantation for micro-CT, histologic, and histomorphometric analyses. The findings obtained were used to calculate new bone volumes (mm3) and new bone areas (%). Excellent cell bioactivity was observed in the PCL/<italic>β</italic>-TCP/bdECM and PCL/<italic>β</italic>-TCP/bdECM/BMP groups, and new bone volume and area were significantly higher in the PCL/<italic>β</italic>-TCP/bdECM/BMP group than in the other groups (p<.05). Within the limitations of this study, bdECM printed PCL/<italic>β</italic>-TCP scaffolds can reproduce microenvironment for cells and promote adhering and proliferating the cells onto scaffolds. Furthermore, in the rat calvarial defect model, the scaffold which printed rhBMP-2 loaded bdECM stably carries rhBMP-2 and enhances bone regeneration confirming the possibility of bdECM as rhBMP-2 carrier.
- Subjects
CELL proliferation; ANIMAL experimentation; BONE morphogenetic proteins; BONE regeneration; BONE growth; CARRIER proteins; EXTRACELLULAR space; PHOSPHATES; POLYESTERS; RATS; RECOMBINANT proteins; CALCIUM compounds; THREE-dimensional printing; IN vitro studies
- Publication
BioMed Research International, 2018, Vol 2018, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2018/2876135