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- Title
Prediction of genetic mutations of equine influenza virus related to adaptive determination nuclear export ribonucleoprotein complex.
- Authors
Boukharta, Mohamed; Kasmi, Yassine; Zakham, Fathiah; El Amri, Hamid; Ennaji, My Mustapha
- Abstract
Background: Segment eight (08) of equine influenza virus strain (H3N8) encodes for two non-structural proteins: NS1 (non-structural protein number 1) and NS2, also called NEP (nuclear export protein), via alternative splicing of the pre-messenger RNA transcripts. The NS1 inhibits the cell defense system, while the NEP activates the translocation of the ribonucleoprotein complex to the cytoplasm, a key step in viral assembly. Mutations in this protein could influence viral multiplication. Results: In this present study, we used computational and in silico tools to investigate the impact of mutations on the three-dimensional structure of the CRM1 of NEP proteins of three equine influenza strains (H3N8) isolated in Morocco (A/equine/Nador/1/1997 (H3N8), A/equine/Essaouira/2/2004 (H3N8), and A/equine/Essaouira/3/2004 (H3N8)). Compared to the reference strain, the two strains of Essaouira showed no mutations in the domain-binding CRM1 of the NEP protein, while the A/equine/Nador/1/1997 strain (H3N8) had two mutations at the T/33/I and Q/34/R residues. These two mutations corresponded to adaptation phenomenon of the equine cellular environment, since they enhance the interaction between the protein NEP and CRM1 proteins with a greater affinity compared to the reference strain. Conclusion: The three-dimensional modification of the NEP protein of the A/equine/Nador/1/1997 strain further facilitates nucleocytoplasmic trafficking in equine species.
- Publication
Journal of Basic & Applied Zoology, 2019, Vol 80, Issue 1, pN.PAG
- ISSN
2090-9896
- Publication type
Article
- DOI
10.1186/s41936-019-0099-x