We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Genomes and virulence difference between two physiological races of Phytophthora nicotianae.
- Authors
Wen Wang; Hui Liu; Xiao Wang; Xiao Ma; Haiqin Yu; Dunhuang Fang; Yongping Li; Bingguang Xiao; Yang Dong
- Abstract
Background: Black shank is a severe plant disease caused by the soil-borne pathogen Phytophthora nicotianae. Two physiological races of, races 0 and 1, are predominantly observed in cultivated tobacco fields around P. nicotianae the world. Race 0 has been reported to be more aggressive, having a shorter incubation period, and causing worse root rot symptoms, while race 1 causes more severe necrosis. The molecular mechanisms underlying the difference in virulence between race 0 and 1 remain elusive. Findings: We assembled and annotated the genomes of P. nicotianae races 0 and 1, which were obtained by a combination of PacBio single-molecular real-time sequencing and second-generation sequencing (both HiSeq and MiSeq platforms). Gene family analysis revealed a highly expanded ATP-binding cassette transporter gene family in P. nicotianae. Specifically, more RxLR effector genes were found in the genome of race 0 than in that of race 1. In addition, RxLR effector genes were found to be mainly distributed in gene-sparse, repeat-rich regions of the P. nicotianae genome. Conclusions: These results provide not only high quality reference genomes of P. nicotianae, but also insights into the infection mechanisms of and its co-evolution with the host plant. They also reveal insights into the P. nicotianae difference in virulence between the two physiological races.
- Subjects
PHYTOPHTHORA nicotianae; GENOMES; MICROBIAL virulence genetics
- Publication
GigaScience, 2016, Vol 5, p1
- ISSN
2047-217X
- Publication type
Article
- DOI
10.1186/s13742-016-0108-7