We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
First Report of Single Nucleotide Polymorphisms (SNPs) of the Leporine Shadow of Prion Protein Gene (SPRN) and Absence of Nonsynonymous SNPs in the Open Reading Frame (ORF) in Rabbits.
- Authors
Memon, Sameeullah; Wang, Zerui; Zou, Wen-Quan; Kim, Yong-Chan; Jeong, Byung-Hoon
- Abstract
Simple Summary: The study focuses on the shadow of prion protein (Sho) encoded by the shadow of prion protein gene (SPRN), which associates with PrP and promotes the progression of prion diseases. While genetic polymorphisms in SPRN are linked to susceptibility in several species, rabbit SPRN gene polymorphisms have not been extensively studied. Through amplicon sequencing, we identified novel single nucleotide polymorphisms (SNPs) in the rabbit SPRN gene and found strong linkage disequilibrium (LD) between them. However, strong LD was not observed between polymorphisms in the prion protein gene (PRNP) and SPRN genes in rabbits. Comparison of amino acid sequences revealed differences in SPRN between rabbits and other species susceptible or resistant to prion diseases. This study represents the first examination of genetic features of the rabbit SPRN gene. Prion disorders are fatal infectious diseases that are caused by a buildup of pathogenic prion protein (PrPSc) in susceptible mammals. According to new findings, the shadow of prion protein (Sho) encoded by the shadow of prion protein gene (SPRN) is associated with prion protein (PrP), promoting the progression of prion diseases. Although genetic polymorphisms in SPRN are associated with susceptibility to several prion diseases, genetic polymorphisms in the rabbit SPRN gene have not been investigated in depth. We discovered two novel single nucleotide polymorphisms (SNPs) in the leporine SPRN gene on chromosome 18 and found strong linkage disequilibrium (LD) between them. Additionally, strong LD was not found between the polymorphisms of PRNP and SPRN genes in rabbits. Furthermore, nonsynonymous SNPs that alter the amino acid sequences within the open reading frame (ORF) of SPRN have been observed in prion disease-susceptible animals, but this is the first report in rabbits. As far as we are aware, this study represents the first examination of the genetic features of the rabbit SPRN gene.
- Subjects
SINGLE nucleotide polymorphisms; PRION diseases; RABBIT diseases; PRIONS; RABBITS; AMINO acid sequence; GENETIC polymorphisms
- Publication
Animals (2076-2615), 2024, Vol 14, Issue 12, p1807
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani14121807