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- Title
Colony Inhibitory Effect of Recombinant Human Tumor Necrosis Factor (rH-TNF) and/or Recombinant Human Interferon (rH-IFN)-α -β and -γ on Human Lung Cancer Cell Lines.
- Authors
HONG, WEON SEON; JETT, JAMES R.; SASAKI, YASUTSUNA; TAKAHASHI, HIDENOBU; NAKANO, HIDEHIKO; NAKAGAWA, KAZUHIKO; HOSHI, AKIO; SAIJO, NAGAHIRO
- Abstract
This study was conducted to assess, by continuous exposure, the inhibitory effects of rH-TNF and rH-IFN-α, -β and -γ, either alone or in combination, on the colony formations of human lung cancer cell lines. The cell lines tested were PC-7 and PC-9 (adenocarcinoma), PC-10 (squamous cell carcinoma) and PC-13 (large cell carcinoma). Additional experiments were performed with L929 (transformed murine fibroblast), because of its known high sensitivity to TNF. rH-TNF inhibited the colony formations of PC-10 and L929 even at a low concentration (10 U/ml). However, PC-7, PC-9 and PC-13 were resistant to rH-TNF. rH-IFN-α, -β and -γ inhibited the colony formation of PC-10 (< 50% of control) at the highest concentration tested (104 U/ml), but only rH-IFN-β inhibited that of PC-7. PC-9, PC-13 and L929 were insensitive to all three rH-IFNs, even at the highest concentrations tested. Combination effects of rH-TNF and rH-IFN-α or -γ were synergistic only at the highest concentration combinations tested in PC-9. However, the maximum inhibition of colony formation of PC-7, PC-9 or PC-13 in any concentration combination treatment was less than 50%.
- Publication
Japanese Journal of Clinical Oncology, 1987, Vol 17, Issue 1, p49
- ISSN
0368-2811
- Publication type
Article