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- Title
High occurrence of Blastocystis sp. subtype 3 in individuals referred to medical laboratories in Kermanshah, Iran.
- Authors
Maleki, Bahman; Sadraei, Javid; Asl, Abdolhossein Dalimi; Pirestani, Majid
- Abstract
Aim: The current study investigated the prevalence and genotypes of Blastocystis sp. in individuals who referred to medical laboratories in Kermanshah, Iran. Background: Blastocystis sp. is a common intestinal protozoan found in humans and a wide range of animals, and it is involved in the development of gastrointestinal disorders. Methods: A total of 950 stool samples were examined using the standard formalin-ether concentration technique. All specimens were cultured in Robinson xenic medium. Subsequently, DNA extraction and PCR amplification of subtype specific sequence-tagged site (STS) were conducted. Results Microscopic examination showed that 86 out of 950 samples (9.05%) were infected with Blastocystis sp. Subsequently, 33 of 86 positive samples were cultured and molecularly confirmed by conventional PCR, indicating six subtypes (ST1-ST6). Of note, ST3 (45.0%) was the predominant subtype, followed by ST1 (15.15%) and ST5 (12%). Conclusion: Based on the current findings, ST3 was the most frequent subtype among all positive samples. Having a better understanding of Blastocystis sp. subtype distribution and risk factors would lead to improved preventive measures.
- Subjects
IRAN; CLASSIFICATION of protozoa; PATHOLOGICAL laboratories; INTESTINAL parasites; GENOTYPES
- Publication
Gastroenterology & Hepatology from Bed to Bench, 2022, Vol 15, Issue 2, p164
- ISSN
2008-2258
- Publication type
Article