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- Title
Sensitive detection of tuberculosis using nanoparticle-enhanced surface plasmon resonance.
- Authors
Chen, Hongxia; Liu, Feng; Koh, Kwangnak; Lee, Jaebeom; Ye, Zonghuang; Yin, Tingting; Sun, Lizhou
- Abstract
We show that the antigen CFP-10 (found in tissue fluids of tuberculosis patients) can be used as a marker protein in a surface-plasmon resonance (SPR) based method for early and simplified diagnosis of tuberculosis. A sandwich SPR immunosensor was constructed by immobilizing the CFP-10 antibody on a self-assembled monolayer on a gold surface, this followed by blocking it with bovine serum albumin. Following exposure of the sensor surface to a sample containing CFP-10, secondary antibody immobilized on nickel oxide nanoparticles are injected which causes a large SPR signal change. The method has a dynamic range from 0.1 to around 150 ng per mL of CFP-10, and a detection limit as low as 0.1 ng per mL. This is assumed to be due to the high amplification power of the NiO nanoparticles. [Figure not available: see fulltext.]
- Subjects
TUBERCULOSIS diagnosis; NANOPARTICLES; SURFACE plasmon resonance; ANTIGENS; BIOMARKERS; MOLECULAR self-assembly; SERUM albumin
- Publication
Microchimica Acta, 2013, Vol 180, Issue 5/6, p431
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-013-0943-5