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- Title
Relationship of mechanical impact magnitude to neurologic dysfunction severity in a rat traumatic brain injury model.
- Authors
Hsieh, Tsung-Hsun; Kang, Jing-Wei; Lai, Jing-Huei; Huang, Ying-Zu; Rotenberg, Alexander; Chen, Kai-Yun; Wang, Jia-Yi; Chan, Shu-Yen; Chen, Shih-Ching; Chiang, Yung-Hsiao; Peng, Chih-Wei
- Abstract
Objective: Traumatic brain injury (TBI) is a major brain injury type commonly caused by traffic accidents, falls, violence, or sports injuries. To obtain mechanistic insights about TBI, experimental animal models such as weight-drop-induced TBI in rats have been developed to mimic closed-head injury in humans. However, the relationship between the mechanical impact level and neurological severity following weight-drop-induced TBI remains uncertain. In this study, we comprehensively investigated the relationship between physical impact and graded severity at various weight-drop heights. Approach: The acceleration, impact force, and displacement during the impact were accurately measured using an accelerometer, a pressure sensor, and a high-speed camera, respectively. In addition, the longitudinal changes in neurological deficits and balance function were investigated at 1, 4, and 7 days post TBI lesion. The inflammatory expression markers tested by Western blot analysis, including glial fibrillary acidic protein, beta-amyloid precursor protein, and bone marrow tyrosine kinase gene in chromosome X, in the frontal cortex, hippocampus, and corpus callosum were investigated at 1 and 7 days post-lesion. Results: Gradations in impact pressure produced progressive degrees of injury severity in the neurological score and balance function. Western blot analysis demonstrated that all inflammatory expression markers were increased at 1 and 7 days post-impact injury when compared to the sham control rats. The severity of neurologic dysfunction and induction in inflammatory markers strongly correlated with the graded mechanical impact levels. Conclusions: We conclude that the weight-drop-induced TBI model can produce graded brain injury and induction of neurobehavioral deficits and may have translational relevance to developing therapeutic strategies for TBI.
- Subjects
BRAIN injuries; LABORATORY rats; TRAFFIC accidents; ACCELERATION (Mechanics); X chromosome
- Publication
PLoS ONE, 2017, Vol 12, Issue 5, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0178186