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- Title
Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control.
- Authors
Hoffmann, Bernd; Blome, Sandra; Bonilauri, Paolo; Fernández-Piñero, Jovita; Greiser-Wilke, Irene; Haegeman, Andy; Isaksson, Mats; Koenen, Frank; LeBlanc, Neil; Leifer, Immanuel; Le Potier, Marie-Frederique; Loeffen, Willie; Rasmussen, Thomas Bruun; Stadejek, Tomasz; Ståhl, Karl; Tignon, Marylène; Uttenthal, Åse; van der Poel, Wim; Beer, Martin
- Abstract
The article discusses a study which describes a ring trial conducted by 10 European laboratories for the detection of Classical swine fever virus (CSFV). Real-time reverse transcription polymerase chain reaction (real-time RT-PCR) protocols and a standardized protocol commonly used by the Friedrich-Loeffler-Institute (FLI) were used for this ring trial. Results of the trial showed that the real-time RT-PCR assay is a rapid and extremely sensitive tool for the detection of CSFV.
- Subjects
EUROPE; CLASSICAL swine fever; POLYMERASE chain reaction; LABORATORIES; RESEARCH institutes
- Publication
Journal of Veterinary Diagnostic Investigation, 2011, Vol 23, Issue 5, p999
- ISSN
1040-6387
- Publication type
Article
- DOI
10.1177/1040638711416849